Guesdon J L, David B, Lapeyre J
Ann Immunol (Paris). 1977 Jul-Oct;128C(4-5):799-810.
Complex antigens were coupled to polyacrylamide agarose beads which had been made magnetic with iron oxide. Those beads were then used in an enzyme-immunoassay for quantitation of specific antibodies to grass-pollen and mites in rabbit antisera. The results obtained by a direct or an inhibition technique proved the specificity of this assay towards homologous antigens. Moreover, cross-reactions between the various species of grass-pollen could be shown. One advantage of this simple method is that it may avoid use of radioactive compounds.