单价阳离子浓度对单核细胞介导的抗体依赖细胞介导的细胞毒性作用的影响。

Influence of monovalent cation concentrations on monocyte-mediated ADCC.

作者信息

Ladisch S, Ulsh L, Feig S A

出版信息

Adv Exp Med Biol. 1982;146:255-64. doi: 10.1007/978-1-4684-8959-0_15.

Abstract

Monocyte-mediated ADCC was inhibited by ouabain, which blocks active transport of Na+ and K+ by the membrane Na+K+-ATPase. Inhibition of ADCC was incomplete, however, even when monovalent cation transport was completely inhibited. On the other hand, increasing the duration of exposure to ouabain did result in complete inhibition of ADCC. ADCC was also depressed by incubation of monocytes in media with low (less than or equal to 1.5 mEq/l) or high (greater than or equal to mEq/l) K+ concentrations. The results suggest that monocyte ADCC is dependent upon monovalent cation concentrations (secondarily altered by ouabain exposure) but not upon active Na+K+ transport per se. Finally, since monovalent cation transport-related glycolysis accounted for essentially all of the previously observed increase in monocyte glycolysis associated with monocyte ADCC, we hypothesize that exposure of monocytes to sensitizing antiserum in the ADCC assay results in increased monocyte membrane permeability to monovalent cations.

摘要

哇巴因可抑制单核细胞介导的抗体依赖细胞介导的细胞毒性作用（ADCC），它能阻断细胞膜上的钠钾ATP酶对Na⁺和K⁺的主动转运。然而，即使单价阳离子转运被完全抑制，ADCC的抑制也不完全。另一方面，延长哇巴因的作用时间确实会导致ADCC被完全抑制。将单核细胞置于低钾（小于或等于1.5 mEq/L）或高钾（大于或等于mEq/L）浓度的培养基中孵育，也会使ADCC受到抑制。结果表明，单核细胞ADCC依赖于单价阳离子浓度（因哇巴因作用而继发改变），而非本身的Na⁺K⁺主动转运。最后，由于与单价阳离子转运相关的糖酵解基本上解释了之前观察到的与单核细胞ADCC相关的单核细胞糖酵解增加的全部情况，我们推测在ADCC试验中，单核细胞暴露于致敏抗血清会导致单核细胞膜对单价阳离子的通透性增加。