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Determination of DNA superhelicity and extremely low levels of DNA strand breaks in low numbers of nonradiolabeled cells by DNA-4',6-diamidino-2-phenylindole fluorescence in nucleoid gradients.

作者信息

Lipetz P D, Brash D E, Joseph L B, Jewett H D, Lisle D R, Lantry L E, Hart R W, Stephens R E

出版信息

Anal Biochem. 1982 Apr;121(2):339-48. doi: 10.1016/0003-2697(82)90491-2.

DOI:10.1016/0003-2697(82)90491-2
PMID:7103066
Abstract
摘要

相似文献

1
Determination of DNA superhelicity and extremely low levels of DNA strand breaks in low numbers of nonradiolabeled cells by DNA-4',6-diamidino-2-phenylindole fluorescence in nucleoid gradients.
Anal Biochem. 1982 Apr;121(2):339-48. doi: 10.1016/0003-2697(82)90491-2.
2
Fluorometric determination of DNA in epidermis and cultured fibroblasts, using 4'-6-diamidino-2-phenylindole (DAPI).使用4′-6-二脒基-2-苯基吲哚(DAPI)对表皮和培养的成纤维细胞中的DNA进行荧光测定。
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Quantitative fluorescent analysis of different conformational forms of DNA bound to the Dye, 4',6-diamidine-2-phenylindole, and separated by gel electrophoresis.对与染料4',6-二脒基-2-苯基吲哚结合并通过凝胶电泳分离的不同构象形式的DNA进行定量荧光分析。
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4
Nucleic acid chemistry: resolution of supercoiled, intact relaxed, and nicked double-stranded DNAs by formamide-sucrose gradients.核酸化学:通过甲酰胺-蔗糖梯度分离超螺旋、完整松弛和带切口的双链DNA
Anal Biochem. 1987 May 15;163(1):200-3. doi: 10.1016/0003-2697(87)90113-8.
5
Characterization of 4'-6 diamidino-2 phenylindole (DAPI) as a fluorescent probe of DNA structure.4'-6-二脒基-2-苯基吲哚(DAPI)作为DNA结构荧光探针的特性研究。
Ital J Biochem. 1982 Mar-Apr;31(2):90-9.
6
Mithramycin- and 4'-6-diamidino-2-phenylindole (DAPI)-DNA staining for fluorescence microspectrophotometric measurement of DNA in nuclei, plastids, and virus particles.用于荧光显微分光光度法测量细胞核、质体和病毒颗粒中DNA的光神霉素和4′-6-二脒基-2-苯基吲哚(DAPI)-DNA染色法。
J Histochem Cytochem. 1981 Aug;29(8):959-68. doi: 10.1177/29.8.6168681.
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Winding anomalies in nuclear DNA from malignant cells. I. Experimental evidence.恶性细胞中核DNA的缠绕异常。I. 实验证据。
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Supercoiling of DNA and nuclear conformation during the cell-cycle.
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Involvement of deoxyribonuclease activity in the differential sedimentation rates of nucleoids from non-transformed and transformed mouse embryo fibroblasts.
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Increasing rates of DNA single-strand breaks in lymphocytes of clinical personnel handling cytostatic drugs.接触细胞毒性药物的临床工作人员淋巴细胞中DNA单链断裂率上升。
J Cancer Res Clin Oncol. 1993;119(4):237-42. doi: 10.1007/BF01624437.
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Distress and DNA repair in human lymphocytes.
J Behav Med. 1985 Dec;8(4):311-20. doi: 10.1007/BF00848366.