Raghunathan R, Wuest C, Faust J, Hwang S, Miller M E
Am J Vet Res. 1982 Aug;43(8):1467-70.
A simple, brief 1-step counterflow centrifugation elutriation technique is described for the recovery of highly purified populations of lymphocytes and granulocytes from adult and fetal ovine peripheral blood. Monocytes are obtained as an enriched population. Lymphocyte and granulocyte recoveries average 80%, higher than with routine gradient centrifugation procedures. In this described technique, inexpensive medium is used, and it is completed in about 90 minutes. The individual blood cellular populations, when tested appropriately, demonstrated normal functional responses.
本文描述了一种简单、简短的一步逆流离心淘析技术，用于从成年和胎儿绵羊外周血中回收高度纯化的淋巴细胞和粒细胞群体。单核细胞作为富集群体获得。淋巴细胞和粒细胞的回收率平均为80%，高于常规梯度离心程序。在所述技术中，使用了廉价的培养基，并且该过程在约90分钟内完成。当进行适当测试时，各个血细胞群体表现出正常的功能反应。
