Pietilä K, Nikkari T
Artery. 1982;10(3):172-9.
When stationary phase cultures of rabbit aortic smooth muscle cells (SMCs) with different cell densities were incubated with [3H] proline in order to study the rates of collagen and total protein syntheses, the incorporation of [3H] proline into collagen and total protein decreased with increasing cell density. This association was mainly due to a negative correlation between cell density and the specific activity of [3H] proline within the free intracellular proline pool. After correction for the specific radioactivity of free intracellular proline, the synthesis rate of collagen was not significantly correlated with cell density, but the negative association between cell density and the incorporation of [3H] proline into total protein was not completely abolished.
为了研究胶原蛋白和总蛋白的合成速率，将不同细胞密度的兔主动脉平滑肌细胞（SMC）的静止期培养物与[3H]脯氨酸一起孵育，结果发现，随着细胞密度的增加，[3H]脯氨酸掺入胶原蛋白和总蛋白的量减少。这种关联主要是由于细胞密度与游离细胞内脯氨酸池内[3H]脯氨酸的比活性呈负相关。在校正游离细胞内脯氨酸的比放射性后，胶原蛋白的合成速率与细胞密度无显著相关性，但细胞密度与[3H]脯氨酸掺入总蛋白之间的负相关并未完全消除。
