乌头酸酶的激活涉及构象变化的证据。
Evidence that the activation of aconitase involves a conformation change.
作者信息
Ramsay R R
出版信息
Biochem J. 1982 Apr 1;203(1):327-30. doi: 10.1042/bj2030327.
Abstract
Reduction of the iron-sulphur cluster of aconitase initiates a slow increase in catalytic activity. It has been proposed that activation involves a conformational change in the protein. Direct evidence for this is presented here in the demonstration that, after reduction of the cluster, the progressive increase in activity is parallelled by an increase in the fluorescence of the protein.
摘要
乌头酸酶铁硫簇的还原引发催化活性的缓慢增加。有人提出，激活涉及蛋白质的构象变化。本文提供了这方面的直接证据，证明在簇还原后，活性的逐渐增加与蛋白质荧光的增加平行。
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引用本文的文献
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Molecular forms of aconitase and their interconversions.乌头酸酶的分子形式及其相互转化
Biochem J. 1984 Jul 15;221(2):489-97. doi: 10.1042/bj2210489.
2
The relationship between the optical properties and the kinetic behaviour of ascorbate-inhibited alkaline phosphatase.抗坏血酸抑制的碱性磷酸酶的光学性质与动力学行为之间的关系。
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本文引用的文献
1
2
Relationship of the oxidation state of the iron-sulfur cluster of aconitase to activity and substrate binding.乌头酸酶铁硫簇的氧化态与活性及底物结合的关系。
Biochemistry. 1981 Dec 22;20(26):7476-82. doi: 10.1021/bi00529a023.
3
Fluorescence spectroscopic investigations of the dynamic properties of proteins, membranes and nucleic acids.蛋白质、膜和核酸动态特性的荧光光谱研究。
J Biochem Biophys Methods. 1980 Jan-Feb;2(1):91-119. doi: 10.1016/0165-022x(80)90077-9.
4
The mechanism of aconitase action. I. Preparation, physical properties of the enzyme, and activation by iron (II).乌头酸酶作用机制。I. 酶的制备、物理性质及铁（II）激活作用
J Biol Chem. 1971 Feb 10;246(3):772-9.
5
Iron and aconitase activity.铁与乌头酸酶活性
Biochem J. 1974 Jun;139(3):709-14. doi: 10.1042/bj1390709.
6
The high potential iron-sulfur cluster of aconitase is a binuclear iron-sulfur cluster.乌头酸酶的高电位铁硫簇是一种双核铁硫簇。
J Biol Chem. 1979 Jun 25;254(12):4967-9.
7
The soluble "high potential" type iron-sulfur protein from mitochondria is aconitase.来自线粒体的可溶性“高电位”型铁硫蛋白是乌头酸酶。
J Biol Chem. 1978 Apr 25;253(8):2514-7.
8
Reversible binding of Pi by beef heart mitochondrial adenosine triphosphatase.牛肉心线粒体三磷酸腺苷酶与磷酸根的可逆结合
J Biol Chem. 1977 May 10;252(9):2891-9.
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