铁与乌头酸酶活性
Iron and aconitase activity.
作者信息
Gawron O, Waheed A, Glaid A J, Jaklitsch A
出版信息
Biochem J. 1974 Jun;139(3):709-14. doi: 10.1042/bj1390709.
Abstract
Aconitase activated with Fe(2+), cysteine and ascorbate incorporates 1 g-atom of Fe(2+)/mol. Loss of this Fe(2+) by transfer to ferrozine, a Fe(2+) chelator, results in loss of activity. Ascorbate increases the rate of transfer of the essential Fe(2+) whereas citrate retards the rate of transfer. Transfer of Fe(2+) from inactive aconitase, 2 g-atoms of Fe/mol, can be accomplished in the presence of urea and ascorbate. The correlation of activity with the presence of an added g-atom of Fe(2+)/mol leads to the conclusion that active aconitase has only one active site per mol.
摘要
用Fe(2+)、半胱氨酸和抗坏血酸激活的乌头酸酶每摩尔结合1克原子的Fe(2+)。通过将这种Fe(2+)转移至Fe(2+)螯合剂亚铁嗪会导致活性丧失。抗坏血酸增加了必需Fe(2+)的转移速率，而柠檬酸盐则减缓了转移速率。在尿素和抗坏血酸存在的情况下，可以实现从无活性的乌头酸酶（每摩尔2克原子的Fe）转移Fe(2+)。活性与每摩尔添加1克原子Fe(2+)的相关性得出结论：活性乌头酸酶每摩尔只有一个活性位点。
相似文献
1
Iron and aconitase activity.铁与乌头酸酶活性
Biochem J. 1974 Jun;139(3):709-14. doi: 10.1042/bj1390709.
2
Iron enzymes in iron deficiency. VI. Aconitase activity and citrate metabolism.缺铁状态下的铁酶。VI. 乌头酸酶活性与柠檬酸代谢。
J Clin Invest. 1959 Sep;38(9):1605-16. doi: 10.1172/JCI103939.
3
The aconitase of yeast. IV. Studies on iron and sulfur in yeast aconitase.酵母顺乌头酸酶。IV。酵母顺乌头酸酶中铁和硫的研究。
J Biochem. 1976 Oct;80(4):799-804. doi: 10.1093/oxfordjournals.jbchem.a131340.
4
Sites and mechanisms of aconitase inactivation by peroxynitrite: modulation by citrate and glutathione.过氧亚硝酸盐使顺乌头酸酶失活的位点和机制：柠檬酸盐和谷胱甘肽的调节作用
Biochemistry. 2005 Sep 13;44(36):11986-96. doi: 10.1021/bi0509393.
5
The aconitase of yeast. II. Crystallization and general properties of yeast aconitase.酵母乌头酸酶。II. 酵母乌头酸酶的结晶及一般性质
J Biochem. 1975 Feb;77(2):367-72. doi: 10.1093/oxfordjournals.jbchem.a130734.
6
Altered aconitase activity in hamster cells selected for resistance to fluorocitrate.在选择对氟柠檬酸具有抗性的仓鼠细胞中，乌头酸酶活性发生改变。
Biochem Biophys Res Commun. 1975 Sep 16;66(2):578-85. doi: 10.1016/0006-291x(75)90549-5.
7
β-Citryl-L-glutamate acts as an iron carrier to activate aconitase activity.β-柠檬酸-L-谷氨酸作为一种铁载体来激活 aconitase 活性。
Biol Pharm Bull. 2011;34(9):1455-64. doi: 10.1248/bpb.34.1455.
8
The aconitase of yeast. V. The reconstitution of yeast aconitase.
J Biochem. 1976 Oct;80(4):805-9. doi: 10.1093/oxfordjournals.jbchem.a131341.
9
AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates.大肠杆菌的AcnC是一种2-甲基柠檬酸脱水酶（PrpD），它可以利用柠檬酸和异柠檬酸作为底物。
Microbiology (Reading). 2002 Jan;148(Pt 1):133-146. doi: 10.1099/00221287-148-1-133.
10
The conversion of citrate into cis-aconitate and isocitrate in the presence of aconitase.在乌头酸酶存在的情况下，柠檬酸转化为顺乌头酸和异柠檬酸。
Biochem J. 1952 Nov;52(3):527-8. doi: 10.1042/bj0520527.
引用本文的文献
1
Biosynthesis of nitric oxide activates iron regulatory factor in macrophages.一氧化氮的生物合成可激活巨噬细胞中的铁调节因子。
EMBO J. 1993 Sep;12(9):3643-9. doi: 10.1002/j.1460-2075.1993.tb06038.x.
2
Evidence that the activation of aconitase involves a conformation change.乌头酸酶的激活涉及构象变化的证据。
Biochem J. 1982 Apr 1;203(1):327-30. doi: 10.1042/bj2030327.
3
Molecular forms of aconitase and their interconversions.乌头酸酶的分子形式及其相互转化
Biochem J. 1984 Jul 15;221(2):489-97. doi: 10.1042/bj2210489.
4
Iron-sulfur stoichiometry and structure of iron-sulfur clusters in three-iron proteins: evidence for [3Fe-4S] clusters.三铁蛋白中铁硫化学计量比及铁硫簇结构：[3Fe-4S]簇的证据
Proc Natl Acad Sci U S A. 1983 Jan;80(2):393-6. doi: 10.1073/pnas.80.2.393.
5
Properties of pig heart aconitase.猪心脏乌头酸酶的特性
Biochem J. 1974 Dec;143(3):717-22. doi: 10.1042/bj1430717.
6
Inhibition of aconitase by alloxan and the differential modes of protection of glucose, 3-O-methylglucose, and mannoheptulose.四氧嘧啶对乌头酸酶的抑制作用以及葡萄糖、3 - O - 甲基葡萄糖和甘露庚酮糖的不同保护模式。
Naunyn Schmiedebergs Arch Pharmacol. 1992 Nov;346(5):532-6. doi: 10.1007/BF00169009.
本文引用的文献
1
The activation of papain by arylamides of mercaptoacetic acid.巯基乙酸芳基酰胺对木瓜蛋白酶的激活作用。
Arch Biochem Biophys. 1951 Nov;34(1):38-49. doi: 10.1016/s0003-9861(51)80006-7.
2
On the determination of serum iron and iron-binding capacity.
Clin Chim Acta. 1958 Nov;3(6):523-30. doi: 10.1016/0009-8981(58)90004-4.
3
The activation of aconitase by ferrous ions and reducing agents.亚铁离子和还原剂对乌头酸酶的激活作用。
Biochem J. 1954 Dec;58(4):685-92. doi: 10.1042/bj0580685.
4
The activation of papain and ficin by phosphorothioate.硫代磷酸酯对木瓜蛋白酶和无花果蛋白酶的激活作用。
Biochemistry. 1967 May;6(5):1421-8. doi: 10.1021/bi00857a026.
5
Mechanism of aconitase action. I. The hydrogen transfer reaction.乌头酸酶作用机制。I. 氢转移反应。
J Biol Chem. 1967 Apr 25;242(8):1870-9.
6
Mechanism of aconitase action deduced from crystallographic studies of its substrates.
J Mol Biol. 1968 Dec 14;38(2):149-62. doi: 10.1016/0022-2836(68)90403-8.
7
Molecular models of metal chelates to illustrate enzymatic reactions.用于阐释酶促反应的金属螯合物分子模型。
J Chem Educ. 1968 Aug;45(8):539-40. doi: 10.1021/ed045p539.
8
Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine).用一种新试剂（亚铁嗪）分光光度法测定亚微克水平的血清铁。
Anal Biochem. 1971 Apr;40(2):450-8. doi: 10.1016/0003-2697(71)90405-2.
9
The mechanism of aconitase action. I. Preparation, physical properties of the enzyme, and activation by iron (II).乌头酸酶作用机制。I. 酶的制备、物理性质及铁（II）激活作用
J Biol Chem. 1971 Feb 10;246(3):772-9.
10
On pig heart aconitase.关于猪心脏顺乌头酸酶。
Biochem Biophys Res Commun. 1972 May 26;47(4):740-5. doi: 10.1016/0006-291x(72)90554-2.
Zotero 插件