精胺刺激大鼠肝脏异亮氨酰 - tRNA 形成的机制。
Mechanism of spermine stimulation of rat-liver isoleucyl-tRNA formation.
作者信息
Igarashi K, Eguchi K, Tanaka M, Hirose S
出版信息
Eur J Biochem. 1978 Sep 15;90(1):13-9. doi: 10.1111/j.1432-1033.1978.tb12569.x.
The mechanism of spermine stimulation of rat-liver isoleucyl-tRNA formation has been studied by Cleland's method [Biochim. Biophys. Acta, 67, 104--137 & 173--197 (1963)]. It was demonstrated that isoleucyl-tRNA formation proceeded through a ping-pong mechanism in the presence of spermine and magnesium. From a study of the effect of spermine on both the formation of isoleucyl-AMP . enzyme complex and on the subsequent formation of isoleucyl-tRNA from the isoleucyl-AMP . enzyme complex, evidence is presented that the stimulatory effect of spermine functions mainly through the spermine preventing the tRNA inhibition of isoleucyl-AMP . enzyme complex formation.
已通过克莱兰方法[《生物化学与生物物理学报》，67, 104 - 137及173 - 197(1963年)]研究了精胺刺激大鼠肝脏异亮氨酰 - tRNA形成的机制。结果表明，在精胺和镁存在的情况下，异亮氨酰 - tRNA的形成通过乒乓机制进行。通过研究精胺对异亮氨酰 - AMP·酶复合物形成以及随后从异亮氨酰 - AMP·酶复合物形成异亮氨酰 - tRNA的影响，有证据表明精胺的刺激作用主要是通过精胺防止tRNA对异亮氨酰 - AMP·酶复合物形成的抑制来发挥的。
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