缬氨霉素对淋巴细胞的作用与钾通透性无关。

Effects of valinomycin on lymphocytes independent of potassium permeability.

作者信息

Negendank W, Shaller C

出版信息

Biochim Biophys Acta. 1982 Jun 14;688(2):316-22. doi: 10.1016/0005-2736(82)90342-x.

Abstract

10(-7) M valinomycin affects human lymphocytes in the following manner: (1) it is non-toxic; (2) it inhibits mitogenesis; (3) it causes a reduction in cell ATP; and (4) it causes a marked increase in steady-state Na+ exchange. However, it has a minimal effect on cell ion (K+, Na+, Ca2+, Mg2+) contents and no effect whatever on K+ exchange. Neither the fast nor the slow fraction of steady-state K+ exchange is affected by 10(-7) M valinomycin. The various reported effects of valinomycin on lymphocyte functions cannot be assumed to be due to changes in plasma membrane K+ permeability. The mechanism of the increase in steady-state Na+ exchange, and whether or not it is related to inhibition of mitogenesis, are unsettled issues.

摘要

10⁻⁷M缬氨霉素对人淋巴细胞的影响如下：（1）它无毒；（2）它抑制有丝分裂；（3）它导致细胞ATP减少；（4）它使稳态钠交换显著增加。然而，它对细胞离子（钾离子、钠离子、钙离子、镁离子）含量的影响极小，对钾离子交换则毫无影响。10⁻⁷M缬氨霉素既不影响稳态钾离子交换的快速部分，也不影响其慢速部分。缬氨霉素对淋巴细胞功能的各种已报道的影响不能被认为是由于质膜钾离子通透性的变化所致。稳态钠交换增加的机制以及它是否与有丝分裂的抑制有关，都是尚未解决的问题。