Samsel J, Zeis A, Weniger J P
Biochimie. 1982 May;64(5):369-76. doi: 10.1016/s0300-9084(82)80442-2.
The question was asked whether the feminization of the chick embryo testis by diethylstilboestrol involves oestrogen secretion. The left gonads of male embryos treated with diethylstilboestrol at the stage of 5 days of incubation were removed at the stage of 17 days and cultured in vitro in the presence of radiolabelled androstenedione, dehydroepiandrosterone, progesterone or sodium acetate. Radiochromatography and repeated crystallizations to constant specific activity were used to demonstrate the formation of oestrone and oestradiol. The results show that oestrogen synthesis takes place from any of the 4 precursors. The normal testis does not produce oestrogens or produces them only in small amounts. Thus, it can be concluded that diethylstilboestrol induces oestrogen synthesis in the chick embryo testis. In a second part of the work, it is shown that tamoxifen antagonizes the feminizing action of diethylstilboestrol on the testes, both at the morphological and biochemical levels.
有人提出问题，己烯雌酚使鸡胚睾丸雌性化是否涉及雌激素分泌。在孵化第5天用己烯雌酚处理的雄性胚胎，在第17天摘除其左侧性腺，并在放射性标记的雄烯二酮、脱氢表雄酮、孕酮或醋酸钠存在的情况下进行体外培养。采用放射色谱法和反复结晶至恒定比活性来证明雌酮和雌二醇的形成。结果表明，雌激素合成可从这4种前体中的任何一种发生。正常睾丸不产生雌激素或仅产生少量雌激素。因此，可以得出结论，己烯雌酚诱导鸡胚睾丸中的雌激素合成。在该研究的第二部分，结果表明，他莫昔芬在形态学和生物化学水平上均拮抗己烯雌酚对睾丸的雌性化作用。
