磁取向叶绿体荧光偏振的场浓度和温度依赖性

Field concentration and temperature dependence of fluorescence polarization of magnetically oriented chloroplasts.

作者信息

Papp E, Meszéna G

出版信息

Biophys J. 1982 Jul;39(1):1-5. doi: 10.1016/S0006-3495(82)84483-4.

DOI:10.1016/S0006-3495(82)84483-4

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1328903/

Abstract

Chloroplasts in higher magnetic fields align with their equatorial plane perpendicular to the field. Because of the nonrandom orientation of the chromophores in the membrane the fluorescence radiation will be partially polarized. The chloroplast concentration, magnetic field, and temperature dependence of the fluorescence polarization has been investigated. The results are compared with a simplified model calculation. It is shown that the concentration dependence can be related to the linear dichroism of the fluorescence radiation and self-adsorption. Taking these effects into account results in the calculation of a higher fluorescence polarization (FP) ratio and higher inclination of chlorophyll dipoles to the membrane plane. Analyzing the magnetic field dependence of the FP ratio, we conclude that in a magnetic field not only will be chloroplasts be aligned, but the thylakoid stacks as well. A decrease in the FP ratio was observed around 20 degrees C. It is suggested that this decrease reflects a phase transition in the photosynthetic membrane.

摘要

在较高磁场中，叶绿体的赤道平面会与磁场垂直排列。由于膜中发色团的非随机取向，荧光辐射会部分偏振。研究了叶绿体浓度、磁场以及荧光偏振的温度依赖性。将结果与简化的模型计算进行了比较。结果表明，浓度依赖性可能与荧光辐射的线性二向色性和自吸收有关。考虑到这些影响，计算得出的荧光偏振（FP）比值更高，且叶绿素偶极子相对于膜平面的倾斜度更大。通过分析FP比值的磁场依赖性，我们得出结论，在磁场中不仅叶绿体，类囊体堆叠也会排列整齐。在20摄氏度左右观察到FP比值下降。有人认为这种下降反映了光合膜中的相变。

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本文引用的文献

1

ON THE MAGNETIC ASYMMETRY OF MUSCLE FIBERS.

Proc Natl Acad Sci U S A. 1958 Jan;44(1):1-4. doi: 10.1073/pnas.44.1.1.

2

The flattening of the absorption spectrum of suspensions, as compared to that of solutions.

Biochim Biophys Acta. 1956 Jan;19(1):1-12. doi: 10.1016/0006-3002(56)90380-8.

3

Orientation of membrane fragments by electric field.

Biochim Biophys Acta. 1980 Jun 6;598(3):429-36. doi: 10.1016/0005-2736(80)90023-1.

4

Orientation of emitting dipoles of chlorophyll A in thylakoids: considerations on the orientation factor in vivo.

Biophys J. 1981 Jun;34(3):423-37. doi: 10.1016/S0006-3495(81)84860-6.

5

Magnetic field induced orientation of photosynthetic systems.

Biochim Biophys Acta. 1972 Apr 20;267(1):65-79. doi: 10.1016/0005-2728(72)90138-7.

6

[Rotation of external segments of photoreceptors in constant magnetic field].

C R Acad Hebd Seances Acad Sci D. 1970 Jul 6;271(1):130-3.

7

Orientation of pigments and structural proteins in the photosynthetic membrane of spinach chloroplasts: a linear dichroism study.

Biochim Biophys Acta. 1973 Jul 26;314(1):42-56. doi: 10.1016/0005-2728(73)90062-5.

8

Polarized light spectroscopy of photosynthetic membranes in magneto-oriented whole cells and chloroplasts. Fluorescence and dichroism.

Biochim Biophys Acta. 1974 Jun 28;347(3):443-63. doi: 10.1016/0005-2728(74)90082-6.

9

Direct observation of the rotation in a constant magnetic field of highly organized lamellar structures.

FEBS Lett. 1975 Feb 1;50(2):257-60. doi: 10.1016/0014-5793(75)80502-3.

10

X-ray diffraction from chloroplast membranes oriented in a magentic field.

FEBS Lett. 1976 Sep 1;67(3):289-93. doi: 10.1016/0014-5793(76)80549-2.

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