脂质过氧化对大鼠肾皮质切片对氨基马尿酸转运的影响。
Effect of lipid peroxidation on p-aminohippurate transport by rat kidney cortical slices.
作者信息
Fujimoto Y, Fujita T
出版信息
Br J Pharmacol. 1982 Jul;76(3):373-9. doi: 10.1111/j.1476-5381.1982.tb09230.x.
1 The effect of lipid peroxidation on p-aminohippurate transport by rat kidney slices was examined. 2 Ascorbic acid and Fe2+ promoted lipid peroxidation of rat renal cortical slices in a dose-related manner. 3 Ascorbic acid (1.0 mM) and Fe2+ (0.4 mM) increased tissue water and decreased the accumulation of p-aminohippurate. 4 The addition of N,N'-diphenyl-p-phenylenediamine (antioxidant), at a concentration of 1 x 10(-6) M, completely inhibited the peroxidation and recovered the accumulation of p-aminohippurate. 5 The apparent Km of p-aminohippurate uptake was increased by ascorbic acid and Fe2+ with no change in the apparent V. 6 These data suggest that ascorbic acid and Fe2+ can cause a significant alteration in p-aminohippurate and water transport of renal cortical slices and that these effects can be correlated with lipid peroxidation.
研究了脂质过氧化对大鼠肾切片对氨基马尿酸转运的影响。
抗坏血酸和Fe2+以剂量相关的方式促进大鼠肾皮质切片的脂质过氧化。
抗坏血酸（1.0 mM）和Fe2+（0.4 mM）增加组织水分并减少对氨基马尿酸的积累。
添加浓度为1×10（-6）M的N，N'-二苯基对苯二胺（抗氧化剂）可完全抑制过氧化并恢复对氨基马尿酸的积累。
抗坏血酸和Fe2+使对氨基马尿酸摄取的表观Km增加，而表观V无变化。
这些数据表明，抗坏血酸和Fe2+可导致肾皮质切片对氨基马尿酸和水转运的显著改变，且这些作用可能与脂质过氧化有关。
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