Singh H, Sethi I
Can J Microbiol. 1982 May;28(5):486-92. doi: 10.1139/m82-074.
Aseptically produced uredospores of race 222 of Puccinia graminis tritici were seeded on defined liquid media containing Czapek's minerals, sucrose or glucose, and various combinations and concentrations of 19 amino acids and a tripeptide, glutathione. The cultures were incubated in the dark at 16--17 degrees C. A medium containing a high concentration of aspartic acid (5988 ppm), cysteine (557 ppm), and glutathione (1014 ppm) supported a profuse growth of the fungus in the form of floating white, fluffy, and vegetative colonies. A sulphur-containing amino acid appears to be essential for the axenic culture of the fungus.
将小麦秆锈菌222号生理小种无菌生产的夏孢子接种于含有察氏矿物质、蔗糖或葡萄糖以及19种氨基酸和一种三肽（谷胱甘肽）的不同组合和浓度的特定液体培养基上。培养物在16 - 17摄氏度的黑暗条件下孵育。一种含有高浓度天冬氨酸（5988 ppm）、半胱氨酸（557 ppm）和谷胱甘肽（1014 ppm）的培养基支持该真菌以漂浮的白色、蓬松的营养菌落形式大量生长。一种含硫氨基酸似乎对该真菌的无菌培养至关重要。
