Gautvik K M, Svindahl K, Skretting A, Stenberg B, Myhre L, Ekeland A, Johannesen J V
Cancer. 1982 Sep 15;50(6):1107-14. doi: 10.1002/1097-0142(19820915)50:6<1107::aid-cncr2820500614>3.0.co;2-j.
A medullary carcinoma of the thyroid gland (MCT) which has been transplanted repeatedly under the kidney capsule of Wag/Rij rats secretes calcitonin (CT) spontaneously. From 10--20 weeks after transplantation, immunoreactive serum calcitonin (iCT) is abnormally elevated and continues to rise parallel to tumor growth. The immunoglobulin fraction of the rabbit anti-CT antiserum raised against intact synthetic hormone, was purified and iodinated electrolytically. Specific activities of 131I-labeled immunoglobulin of 0.008--0.014 mCi/microgram protein were obtained with 80% preservation of CT binding activity. Wag/Rig rats with MCT tumor and increased serum iCT concentrations received intravenous injections of 131I-labeled immunoglobulins (0.054--0.811 mCi). The distribution of radioactivity in the rats was followed for 14 days using external scintigraphy in combination with radioactivity measurements of blood and different organs at the end of the observation period. The distribution of 113mIn was used as a marker for blood distribution. When the radioactivity ratios (131I/113mIn) in tumor and different organs were related to that of blood which was set equal to unity, tumor tissue contained 3--6 times higher activity. Nonhyperimmune rabbit immunoglobulins or rabbit antirat prolactin immunoglobulins were not concentrated in MCT tissue, nor did anti-CT immunoglobulins localize in rat prolactin adenomas.
一种已在Wag/Rij大鼠肾被膜下反复移植的甲状腺髓样癌（MCT）能自发分泌降钙素（CT）。移植后10至20周，免疫反应性血清降钙素（iCT）异常升高，并随着肿瘤生长持续上升。用完整的合成激素免疫家兔制备的抗CT抗血清，其免疫球蛋白部分经纯化后进行电解碘化。获得的131I标记免疫球蛋白的比活为0.008至0.014mCi/μg蛋白，且CT结合活性保留了80%。患有MCT肿瘤且血清iCT浓度升高的Wag/Rig大鼠静脉注射131I标记的免疫球蛋白（0.054至0.811mCi）。在观察期结束时，结合外部闪烁扫描以及对血液和不同器官的放射性测量，追踪大鼠体内放射性分布14天。113mIn的分布用作血液分布的标记。当肿瘤和不同器官中的放射性比率（131I/113mIn）与设定为1的血液中的比率相关时，肿瘤组织中的活性高3至6倍。非免疫家兔免疫球蛋白或兔抗大鼠催乳素免疫球蛋白不会在MCT组织中聚集，抗CT免疫球蛋白也不会定位于大鼠催乳素腺瘤中。
