Yoo T J, Kuo C Y, Spector A A, Denning G M, Floyd R, Whiteaker S, Kim H, Kim J, Abbas M, Budd T W
Cancer Res. 1982 Sep;42(9):3596-600.
Rat hepatoma (Morris 7777) cells modified with either oleic or linoleic acid exhibited greater susceptibility to normal spleen cell-mediated lysis in a 16-hr 51Cr release assay. At effector:target cell ratios of 300:1, the specific lysis of fatty acid-enriched target cells (cultured for 2 days in fatty acid-supplemented medium) by the normal rat spleen cells was 60% higher than the untreated target cells (P less than 0.01). Prolonging the culture in fatty acid-supplemented medium up to 6 days produced similar effects. Analysis of the fatty acid composition of cellular lipids revealed that an elevation of oleic or linoleic acid was the only significant alteration in the hepatoma cells grown in the oleic or linoleic acid-supplemented medium, respectively. The percentage of the acids was increased in the total cellular phospholipids, the choline, ethanolamine, serine, and inositol phosphoglyceride fractions, and the neutral lipids. In conclusion, we suggest that the elevation of oleic acid and linoleic acid contents in the membranes of the fatty acid-modified hepatoma cells may contribute to the increased susceptibility of these cells to natural killer cell-mediated cytotoxicity.
在一项16小时的51铬释放试验中，用油酸或亚油酸修饰的大鼠肝癌（莫里斯7777）细胞对正常脾细胞介导的裂解表现出更高的敏感性。在效应细胞与靶细胞比例为300:1时，正常大鼠脾细胞对富含脂肪酸的靶细胞（在补充脂肪酸的培养基中培养2天）的特异性裂解比未处理的靶细胞高60%（P小于0.01）。在补充脂肪酸的培养基中延长培养至6天产生了类似的效果。对细胞脂质的脂肪酸组成分析表明，在分别添加油酸或亚油酸的培养基中生长的肝癌细胞中，油酸或亚油酸的升高是唯一显著的变化。这些酸在总细胞磷脂、胆碱、乙醇胺、丝氨酸和肌醇磷酸甘油酯部分以及中性脂质中的百分比增加。总之，我们认为脂肪酸修饰的肝癌细胞膜中油酸和亚油酸含量的升高可能导致这些细胞对自然杀伤细胞介导的细胞毒性的敏感性增加。
