Martin L H, Corff S C, Yuyama S
Cytobios. 1982;33(129):29-38.
Naegleria gruberi amoebae were stimulated to differentiate synchronously into flagellates under growth conditions by lowering the temperature from 32 degrees C to 20.5 degrees C. In the presence of nutrient medium, flagellates will eventually revert to amoebae and resume growth. The time course of nuclear DNA synthesis, using a double thymidine isotope procedure, was determined for: (1) logarithmically growing amoebae, (2) differentiating cells, and (3) flagellates that were reverting to amoebae. DNA replication ceased 10 min. after the stimulation of differentiation, and began again during reversion. Neither de novo transcription nor translation appear to be required for the cessation of DNA replication during differentiation.
通过将温度从32摄氏度降至20.5摄氏度，刺激格氏耐格里变形虫在生长条件下同步分化为鞭毛虫。在营养培养基存在的情况下，鞭毛虫最终会恢复为变形虫并重新开始生长。使用双胸苷同位素程序确定了以下细胞的核DNA合成时间进程：（1）对数生长期的变形虫，（2）分化中的细胞，以及（3）正在恢复为变形虫的鞭毛虫。DNA复制在分化刺激后10分钟停止，并在恢复过程中再次开始。分化过程中DNA复制的停止似乎既不需要从头转录也不需要翻译。
