Metivier H J, Masse R, Vai F, Pariente R G
Exp Lung Res. 1982 May;3(2):129-36. doi: 10.3109/01902148209063287.
Hydroxyproline transfer to an incubation medium was studied in ten emphysematous, nine relapsing pneumothorax, and eight control samples surgically removed from 2 females and 25 males, ranging in age from 17 to 69 years. Total hydroxyproline in the lung pieces, expressed as a fraction of DNA, was not modified. A large increase of dialyzable and nondialyzable hydroxyproline fraction was observed in the incubation medium of biopsies from emphysema dn pneumothorax groups. Although the pneumothorax group had average values between controls and emphysema, some pneumothorax patients exhibited very high content of soluble hydroxyproline, suggesting a common pattern between some relapsing pneumothorax and emphysema. These findings could not be due to extracellular degradation during the processing of the samples since the results were not affected by the presence of protease inhibitors. These observations suggest that individuals with emphysema and pneumothrax have abnormalities in lung collagen structure and/or biosynthesis resulting in increased degradation of this macromolecule.
研究了从2名女性和25名男性（年龄在17至69岁之间）手术切除的10份肺气肿、9份复发性气胸和8份对照样本中羟脯氨酸向孵育培养基的转移情况。肺组织块中的总羟脯氨酸，以DNA的分数表示，未发生改变。在肺气肿组和气胸组活检样本的孵育培养基中，可透析和不可透析的羟脯氨酸分数均大幅增加。尽管气胸组的平均值介于对照组和肺气肿组之间，但一些气胸患者的可溶性羟脯氨酸含量非常高，这表明一些复发性气胸和肺气肿之间存在共同模式。这些发现不可能是由于样本处理过程中的细胞外降解所致，因为结果不受蛋白酶抑制剂存在的影响。这些观察结果表明，患有肺气肿和气胸的个体在肺胶原结构和/或生物合成方面存在异常，导致这种大分子的降解增加。
