Kowalczyńska H M, Nowak M, Dowjat W K, Doroszewski J
J Cell Sci. 1982 Jun;55:317-25. doi: 10.1242/jcs.55.1.317.
Examination was made of the adhesive interaction of L5222 leukemia cells with endothelial cells, collagen and glass and of cell locomotion on endothelium and collagen. Leukaemia cells interacted with the substrate under stationary conditions. The fraction of cells adherent to the substrate was defined next, using the given shearing force caused by the medium flowing through the measuring channel. The relative number of adherent cells, A (related to the number of cells after sedimentation), remaining on the given surface despite the detaching action of shearing force, F, was determined. The range of F values applied was 0.1 to 30 (x 10(-13)) N. It was found that the relation A(F) is a decreasing function for all the substrates examined and takes on values in the relation 1:2:6 for cells adherent to collagen, glass and endothelium, respectively. The critical value of F, at which values of A are maintained at a constant level close to zero, was 0.5, 1 and 3 (x 10(12)) N, respectively, for cells adherent to these substrates. The function A(F) for L5222 cells adherent to endothelial cells and to glass can be described well by the formula: A = a/square root F (where a = constant greater than 0). Studies of L5222 cell locomotion on an endothelial cell layer and on collagen revealed that the pattern of locomotion, variations in locomotion velocity and the mean values of cell displacement (5.8 and 5.0 micrometers, respectively) are similar for both substrates.
对L5222白血病细胞与内皮细胞、胶原蛋白和玻璃之间的黏附相互作用以及细胞在内皮和胶原蛋白上的运动进行了检测。白血病细胞在静止条件下与底物相互作用。接下来，利用流经测量通道的培养基产生的给定剪切力，确定附着在底物上的细胞比例。确定了在剪切力F的分离作用下，仍留在给定表面上的附着细胞的相对数量A（相对于沉降后的细胞数量）。所施加的F值范围为0.1至30（×10⁻¹³）N。结果发现，对于所有检测的底物，A(F)关系均为递减函数，对于附着在胶原蛋白、玻璃和内皮上的细胞，其取值比例分别为1:2:6。对于附着在这些底物上的细胞，A保持在接近零的恒定水平时的临界F值分别为0.5、1和3（×10¹²）N。附着在内皮细胞和玻璃上的L5222细胞的A(F)函数可用公式A = a/√F（其中a为大于0的常数）很好地描述。对L5222细胞在内皮细胞层和胶原蛋白上运动的研究表明，两种底物上的运动模式、运动速度变化以及细胞位移的平均值（分别为5.8和5.0微米）相似。
