Krause W, Jakobs U
J Chromatogr. 1982 Jun 11;230(1):37-45. doi: 10.1016/s0378-4347(00)81428-0.
A method for the determination of plasma concentrations of spirorenone, a new aldosterone antagonist, and one of its metabolites, chromatographically characterized as 1,2-dihydro-spirorenone, is described. The assay utilizes high-performance liquid chromatography with UV detection. Reproducible results can be obtained with standard deviations of about 5% and the limit of detection is less than 5 ng/ml. Plasma levels of drug and metabolite have been measured after oral doses of 10 and 40 mg, respectively, administered to two male volunteers.
本文描述了一种测定新型醛固酮拮抗剂螺内酯及其一种代谢物（经色谱鉴定为1,2 - 二氢螺内酯）血浆浓度的方法。该测定采用高效液相色谱 - 紫外检测法。可获得重现性良好的结果，标准偏差约为5%，检测限小于5 ng/ml。分别向两名男性志愿者口服10 mg和40 mg剂量后，测定了药物和代谢物的血浆水平。
