采用反相液相色谱法快速测定血浆苯丁酸氮芥和苯乙酸氮芥

Rapid assay for plasma chlorambucil and phenyl acetic mustard using reversed-phase liquid chromatography.

作者信息

Zakaria M, Brown P R

出版信息

J Chromatogr. 1982 Jul 9;230(2):381-9. doi: 10.1016/s0378-4347(00)80488-0.

Abstract

A rapid assay for chlorambucil, a drug used for the treatment of chronic lymphocytic leukemia, and its major metabolite is described. Chromatographic behaviour of the two compounds on two different reversed-phase columns is discussed, as well as the kinetics of their hydrolysis in aqueous medium. The developed analysis can be applied to the determination of the plasma levels of the drug and its metabolite. No sample preparation is required and the spectrophotometric detection affords the sensitivity in the picomole range. Total analysis time is between 10 and 15 min.

摘要

本文描述了一种用于治疗慢性淋巴细胞白血病的药物苯丁酸氮芥及其主要代谢物的快速检测方法。讨论了这两种化合物在两种不同反相柱上的色谱行为，以及它们在水介质中的水解动力学。所开发的分析方法可用于测定药物及其代谢物的血浆水平。无需样品制备，分光光度检测在皮摩尔范围内提供灵敏度。总分析时间在10至15分钟之间。