高效液相色谱法测定生物样品中的阿朴长春胺酸

Determination of apovincaminic acid in biological samples by high-performance liquid chromatography.

作者信息

Kozma M, Pudleiner P, Vereczkey L

出版信息

J Chromatogr. 1982 May 28;241(1):177-82. doi: 10.1016/s0021-9673(00)82402-4.

Abstract

A method is described for measuring the concentrations of apovincaminic acid and vincaminic acid (internal standard) in blood plasma and urine by high-performance liquid chromatography using ion pair extraction. The main metabolite of vinpocetine, apovincaminic acid, and vincaminic acid were extracted from 1 ml of plasma and urine into chloroform as an ion pair using tetrabutylammonium hydroxide as counter ion. Analysis was carried out on a reversed-phase column of RP-8 with acetonitrile-0.0075 M phosphate buffer (28:72) at pH 3.5 as mobile phase. The eluted derivatives were detected by UV absorption at 254 nm. The sensitivity of the method is 20 ng/ml for AVA in plasma and urine samples. The relative recovery of these compounds added to plasma was about 50%.

摘要

本文描述了一种采用离子对萃取的高效液相色谱法测定血浆和尿液中阿朴长春胺酸和长春胺酸（内标）浓度的方法。从1毫升血浆和尿液中，以氢氧化四丁铵作为反离子，将长春西汀的主要代谢产物阿朴长春胺酸和长春胺酸作为离子对萃取至氯仿中。分析在RP - 8反相柱上进行，以乙腈 - 0.0075M磷酸盐缓冲液（28:72），pH 3.5作为流动相。洗脱的衍生物通过254nm处的紫外吸收进行检测。该方法对血浆和尿液样本中阿朴长春胺酸的灵敏度为20 ng/ml。添加到血浆中的这些化合物的相对回收率约为50%。