Westergaard L, McNatty K P, Christensen I, Larsen J K, Byskov A G
J Clin Endocrinol Metab. 1982 Oct;55(4):693-8. doi: 10.1210/jcem-55-4-693.
Granulosa cell aspirates from human ovarian follicles were analyzed by flow cytometry to determine the fraction of cells in the DNA S-phase of the mitotic cell cycle. The aim of the study was to evaluate if the percentage of granulosa cells in S-phase (the S-fraction) could be used to indicate whether a follicle was healthy or atretic. A highly significant relationship was found between the S-fraction and the concentration of estradiol in the follicular fluid (r = 0.6, P less than 0.001). More than 85% of the follicles having an S-fraction of 16% or greater contained intrafollicular levels of estradiol equal to or greater than 200 ng/ml and had a low androstenedione:estradiol ratio. Conversely, 95% or more of the follicles that had an S-fraction of less than 16% contained low estradiol (less than 200 ng/ml) and had a high androstenedione to estradiol ratio. We conclude that flow cytometric DNA measurements on follicular aspirates provide a reliable and rapid method by which to distinguish healthy and atretic ovarian follicles. Since only a small fraction (less than 5%) of an entire granulosa cell population is required for S-phase analysis, the technique allows the majority of cells to be immediately available or other biochemical studies. Moreover, since excision of ovarian tissue is avoided, the technique may be acceptable for studies on women with normal ovarian function but who are undergoing laparotomy or laparoscopy for some reason.
通过流式细胞术分析人卵巢卵泡的颗粒细胞抽吸物，以确定有丝分裂细胞周期中处于DNA合成期（S期）的细胞比例。本研究的目的是评估S期颗粒细胞百分比（S比例）是否可用于指示卵泡是健康的还是闭锁的。发现S比例与卵泡液中雌二醇浓度之间存在高度显著的相关性（r = 0.6，P < 0.001）。S比例为16%或更高的卵泡中，超过85%的卵泡内雌二醇水平等于或大于200 ng/ml，且雄烯二酮：雌二醇比值较低。相反，S比例小于16%的卵泡中，95%或更多的卵泡雌二醇水平较低（小于200 ng/ml），且雄烯二酮与雌二醇比值较高。我们得出结论，对卵泡抽吸物进行流式细胞术DNA测量提供了一种可靠且快速的方法，可用于区分健康和闭锁的卵巢卵泡。由于S期分析仅需要整个颗粒细胞群体的一小部分（小于5%），该技术使得大多数细胞可立即用于其他生化研究。此外，由于避免了卵巢组织的切除，该技术对于卵巢功能正常但因某种原因正在接受剖腹手术或腹腔镜检查的女性的研究可能是可接受的。
