Kasper L H, Crabb J H, Pfefferkorn E R
J Immunol. 1982 Oct;129(4):1694-9.
Several monoclonal antibodies against Toxoplasma gondii were parasiticidal in the presence of normal human serum as measured by reduction in plaque titer or in assays that detected lysis. One monoclonal antibody, G, was used to select a resistant mutant from a large population of chemically mutagenized wild-type parasites. This mutant retained the wild-type sensitivity to other monoclonal antibodies and to polyclonal antisera. A comparison of surface radioiodinated wild-type and mutant parasites by using one and two-dimensional electrophoresis showed that the mutant lacked a protein (or proteins) of approximate m.w. 22,000. An immunoprecipitation procedure using monoclonal antibody G yielded a protein(s) of this m.w. from surface radioiodinated wild-type T. gondii but not from surface radioiodinated mutant parasites.
通过噬斑效价降低或检测裂解的试验测定，几种抗刚地弓形虫的单克隆抗体在正常人血清存在的情况下具有杀寄生虫作用。一种单克隆抗体G被用于从大量经化学诱变的野生型寄生虫群体中筛选出一个抗性突变体。该突变体对其他单克隆抗体和多克隆抗血清仍保留野生型敏感性。通过一维和二维电泳对表面放射性碘化的野生型和突变型寄生虫进行比较，结果显示该突变体缺乏一种分子量约为22,000的蛋白质。使用单克隆抗体G的免疫沉淀程序从表面放射性碘化的野生型刚地弓形虫中获得了该分子量的一种蛋白质，但从表面放射性碘化的突变型寄生虫中未获得。
