Zambryski P, Depicker A, Kruger K, Goodman H M
J Mol Appl Genet. 1982;1(4):361-70.
Molecular cloning has been used to isolate the ends of that portion of the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens which has been designated T-DNA and which has been transferred to the genome of tobacco crown gall tumor cells. Analysis of the DNA sequences of the plant border clones compared with the corresponding sequences of the Ti plasmid suggests that the mechanism of transferred DNA integration and subsequent stabilization is precise at the right border and imprecise on the left. The T-DNA junction occurs within a variation of a single base pair (bp) on the right but varies over at least 70 bp on the left. In addition, there are several sequences which are repeated near the ends of the T-DNA region in the Ti plasmid. Seemingly, there is no specificity with regard to the site of integration in the plant genome.
分子克隆技术已被用于分离根癌土壤杆菌肿瘤诱导（Ti）质粒中被称为T-DNA的那部分的末端，该部分已转移到烟草冠瘿瘤细胞的基因组中。将植物边界克隆的DNA序列与Ti质粒的相应序列进行比较分析表明，转移DNA整合及随后稳定化的机制在右边界是精确的，而在左边界是不精确的。T-DNA连接在右侧单个碱基对（bp）的变化范围内发生，但在左侧至少有70 bp的变化。此外，在Ti质粒的T-DNA区域末端附近有几个序列是重复的。显然，植物基因组中的整合位点没有特异性。
