Byrne M C, Koplow J, David C, Tempé J, Chilton M D
J Mol Appl Genet. 1983;2(2):201-9.
DNA isolated from hairy roots incited on Daucus carota by Agrobacterium strains harboring the Ri plasmid was probed with Ri plasmid fragments to identify plasmid sequences transferred and integrated into the plant genome. One hairy root line was incited by an A. rhizogenes natural isolate strain harboring two large plasmids in addition to the Ri plasmid. DNA from hairy roots incited by this strain contained a total of five nonidentical copies of T-DNA which together comprised a T-DNA complement representing a 34-42 kb segment of the Ri plasmid. A second hairy root line was incited by a Ti plasmidless A. tumefaciens strain into which the Ri plasmid had been conjugated. Hairy root DNA incited by this strain contained four identical T-DNA copies 17-18 kb in length and one truncated copy. T-DNA borders occurred at preferential sites of the Ri plasmid. T-DNA structure in these hairy root lines is similar to T-DNA structure in tumors incited by Ti plasmids of A. tumefaciens.
用携带Ri质粒的农杆菌菌株在胡萝卜上诱导产生的毛状根中分离出的DNA，用Ri质粒片段进行探测，以鉴定转移并整合到植物基因组中的质粒序列。一个毛状根系由发根农杆菌自然分离株诱导产生，该菌株除了Ri质粒外还含有两个大质粒。由该菌株诱导产生的毛状根的DNA总共包含五个不相同的T-DNA拷贝，它们共同构成了一个T-DNA互补体，代表了Ri质粒的一个34-42 kb片段。第二个毛状根系由一个无Ti质粒的根癌农杆菌菌株诱导产生，Ri质粒已与该菌株进行了接合。由该菌株诱导产生的毛状根DNA包含四个长度为17-18 kb的相同T-DNA拷贝和一个截短拷贝。T-DNA边界出现在Ri质粒的优先位点。这些毛状根系中的T-DNA结构与根癌农杆菌Ti质粒诱导产生的肿瘤中的T-DNA结构相似。
