Hammerman K J, Chen L J, Fernandez-Corugedo A, Earnest D L
Gastroenterology. 1978 Dec;75(6):1021-5.
Metabolism of the bile salts by formation of sulfate esters is catalyzed by bile salt sulfotransferase, an enzyme isolated from rat liver and kidney. The activity of bile salt sulfotransferase was measured in liver and kidney of male and female rats and in oophorectomized rats with or without estrogen replacement. In vitro sulfotransferase activity was correlated with in vivo sulfation by measuring the percentage of an infused dose (0.03 micron per 100 g per min) of taurolithocholate, which was excreted in bile as the sulfate. The activity of sulfotransferase in liver was higher in females (26.3 +/- 3.0 pmoles per mg of protein per min) than in males (9.6 +/- 3.9) and was lower (12.1 +/- 3.8) after oophorectomy. The decrease in activity was prevented by replacement of estrogen. Renal sulfotransferase activity did not differ between the sexes and was unaffected by oophorectomy. Hepatic sulfotransferase activity measured in vitro correlated with in vivo sulfation of taurolithocholate. This study shows definite sex differences in hepatic bile salt sulfotransferase activity, which in females is affected by the presence of estrogen. The correlation between in vitro sulfotransferase activity and in vivo bile salt sulfation suggests that bile salt sulfotransferase is responsible for bile salt sulfation in vivo.
胆盐硫酸酯的形成所介导的胆盐代谢由胆盐磺基转移酶催化，该酶是从大鼠肝脏和肾脏中分离出来的一种酶。在雄性和雌性大鼠的肝脏和肾脏以及接受或未接受雌激素替代的卵巢切除大鼠中测量了胆盐磺基转移酶的活性。通过测量注入剂量（每100克每分钟0.03微米）的牛磺石胆酸盐以硫酸盐形式随胆汁排出的百分比，体外磺基转移酶活性与体内硫酸化相关。肝脏中磺基转移酶的活性在雌性大鼠中（每毫克蛋白质每分钟26.3±3.0皮摩尔）高于雄性大鼠（9.6±3.9），在卵巢切除后降低（12.1±3.8）。雌激素替代可防止活性降低。肾脏磺基转移酶活性在两性之间没有差异，并且不受卵巢切除的影响。体外测量的肝脏磺基转移酶活性与牛磺石胆酸盐的体内硫酸化相关。这项研究表明肝脏胆盐磺基转移酶活性存在明显的性别差异，在雌性中该活性受雌激素的影响。体外磺基转移酶活性与体内胆盐硫酸化之间的相关性表明胆盐磺基转移酶负责体内胆盐的硫酸化。
