噬菌体T7 DNA的体外包装需要ATP。
In vitro packaging of bacteriophage T7 DNA requires ATP.
作者信息
Masker W E
出版信息
J Virol. 1982 Jul;43(1):365-7. doi: 10.1128/JVI.43.1.365-367.1982.
Abstract
Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.
摘要
从感染了噬菌体T7的大肠杆菌制备的提取物中去除三磷酸核苷，会导致在通过体外包装噬菌体T7 DNA形成感染性噬菌体颗粒时，对添加的ATP有严格需求。在约1.25 mM的浓度下可实现最佳包装效率。其他三磷酸核苷可替代ATP，但在促进体外封装方面，常见的三磷酸核苷都不如ATP有效。
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