通过分离的噬菌体T7原衣壳在体外进行DNA包装。
DNA packaging in vitro by an isolated bacteriophage T7 procapsid.
作者信息
Masker W E, Serwer P
出版信息
J Virol. 1982 Sep;43(3):1138-42. doi: 10.1128/JVI.43.3.1138-1142.1982.
Abstract
The results of previous in vivo studies indicate that a DNA-free procapsid (capsid I) packages bacteriophage T7 DNA during infection of Escherichia coli. It was shown here that capsid I, isolated by electrophoresis in metrizamide density gradients, packaged DNA and formed infectious phage particles when incubated in vitro with extracts deficient in capsid proteins.
摘要
先前的体内研究结果表明，无DNA的原衣壳（衣壳I）在大肠杆菌感染期间包装噬菌体T7 DNA。此处表明，通过在甲泛葡胺密度梯度中进行电泳分离得到的衣壳I，在体外与衣壳蛋白缺陷提取物一起孵育时，能够包装DNA并形成有感染性的噬菌体颗粒。
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