经Percoll密度梯度分离的苯肼注射大鼠红细胞的变形性和渗透脆性

Deformability and osmotic fragility of phenylhydrazine-injected rat erythrocytes fractionated by Percoll density-gradients.

作者信息

Nagasawa T

出版信息

Jpn J Physiol. 1982;32(2):161-70. doi: 10.2170/jjphysiol.32.161.

DOI:10.2170/jjphysiol.32.161

Abstract

Red blood cells (RBCs) from phenylhydrazine-injected rats were separated according to their specific densities by centrifugation on a polyvinyl-pyrrolidone-coated colloidal silica matrix (Percoll). The deformability and osmotic fragility of fractionated RBCs were measured by ektacytometer and coil planet centrifuge, respectively. Immature reticulocytes, the least dense RBC subpopulation, were less deformable and osmotically more resistant than normal RBCs. Heinz-body containing RBC, the most dense subpopulation, showed reduced deformability and increased osmotic fragility. These findings suggest that young reticulocytes have increased membrane stiffness and the Heinz-body containing RBCs have an extremely rigid membrane.

摘要

通过在聚乙烯吡咯烷酮包被的胶体二氧化硅基质（Percoll）上离心，根据其特定密度分离苯肼注射大鼠的红细胞（RBC）。分别通过血细胞变形仪和盘管行星离心机测量分级分离的红细胞的变形性和渗透脆性。未成熟网织红细胞是密度最低的红细胞亚群，其变形性比正常红细胞低，对渗透压的抵抗力更强。含亨氏小体的红细胞是密度最高的亚群，其变形性降低，渗透脆性增加。这些发现表明，年轻网织红细胞的膜硬度增加，而含亨氏小体的红细胞具有极其刚性的膜。