Sutherland C M, Leong S P, Cooperband S R, Deckers P J, Hornung M O, Krementz E T
J Surg Oncol. 1982 Aug;20(4):221-6. doi: 10.1002/jso.2930200407.
Sera from patients receiving autologous cell immunization were shown to contain antibody specific for melanoma cells by immunofluorescence, microcomplement fixation, and mixed hemadsorption. These hyperimmune specific sera were used to define antigens shed into tissue culture media from human malignant melanoma cells. When supernatants from cultured melanoma cells were passed over a Sephadex G-200 column, melanoma antigens were found in Peak 2. The active fraction was further purified by carboxymethylcellulose ion exchanger and found in the 0.4 M NaCl gradient. This fraction has been tested in four immunized patients and in one nonimmunized melanoma patient. All five patients developed lymphocytic vasculitis compatible with delayed hypersensitivity reaction. These antigens should be of benefit in studying delayed hypersensitivity in melanoma patients and as potential agents for specific immunotherapy.
通过免疫荧光、微量补体结合和混合血细胞吸附试验表明，接受自体细胞免疫的患者血清中含有对黑色素瘤细胞具有特异性的抗体。这些超免疫特异性血清被用于鉴定从人恶性黑色素瘤细胞释放到组织培养基中的抗原。当培养的黑色素瘤细胞的上清液通过葡聚糖G - 200柱时，在峰2中发现了黑色素瘤抗原。活性组分通过羧甲基纤维素离子交换剂进一步纯化，并在0.4M NaCl梯度中被发现。该组分已在4名免疫患者和1名未免疫的黑色素瘤患者中进行了测试。所有5名患者均出现了与迟发型超敏反应相符的淋巴细胞性血管炎。这些抗原在研究黑色素瘤患者的迟发型超敏反应以及作为特异性免疫治疗的潜在药物方面应具有益处。
