Felíu J E, Coloma J, Gómez-Lechón M J, García M D, Báguena J
Mol Cell Biochem. 1982 Jun 11;45(2):73-81. doi: 10.1007/BF00223502.
The influence of dexamethasone on the isozyme patterns of ATP-hexose phosphotransferases, aldolase and pyruvate kinase of adult rat hepatocytes maintained in primary cultures has been studied. A progressive loss of the typical adult liver isozymes glucokinase, pyruvate kinase L and aldolase B, with a simultaneous increase of both pyruvate kinase A and hexokinase activities, was observed in hepatocytes cultured in the absence of added glucocorticoid. When the culture medium was supplemented with 10(-7)M dexamethasone, the adult liver patterns of pyruvate kinase and aldolase were preserved for at least seven days of culture, the initial level of glucokinase was maintained for three days, and the rise of hexokinase activity was delayed and partially blocked. These results are discussed in relation to the known beneficial effect of glucocorticoids on the survival of cultured hepatocytes.
研究了地塞米松对原代培养的成年大鼠肝细胞中ATP - 己糖磷酸转移酶、醛缩酶和丙酮酸激酶同工酶模式的影响。在未添加糖皮质激素的培养基中培养的肝细胞中，观察到典型的成年肝脏同工酶葡萄糖激酶、丙酮酸激酶L和醛缩酶B逐渐丧失，同时丙酮酸激酶A和己糖激酶活性均增加。当培养基中添加10(-7)M地塞米松时，丙酮酸激酶和醛缩酶的成年肝脏模式在至少七天的培养中得以保留，葡萄糖激酶的初始水平维持三天，己糖激酶活性的升高被延迟并部分受阻。结合糖皮质激素对培养肝细胞存活的已知有益作用对这些结果进行了讨论。
