Guguen-Guillouzo C, Szajnert M F, Glaise D, Gregori C, Schapira F
In Vitro. 1981 May;17(5):369-77. doi: 10.1007/BF02626734.
Aldolase and pyruvate kinase isozymes were investigated in cultured hepatocytes from fetal, regenerating, and 2-acetyl-aminofluorene-fed rat liver as well as in some epithelial liver cell lines. Our results show that: (a) cell proliferation and prolonged expression of specific isozymes were found only in cultured hepatocytes from 17-day old fetuses; (b) the fetal type of pyruvate kinase expressed in regenerating and carcinogen-treated liver was temporarily lost only in cultured hepatocytes from regenerating liver; (c) the adult type of aldolase and pyruvate kinase was absent in one epithelial cell line derived from a carcinogen-treated liver and in the hepatoma tissue cell (HTC) line but was found in the Faza clone of the Reuber H35 cell line during the 50 first passages in vitro; and (d) the isozyme pattern of pyruvate kinase was always more strongly shifted than that of aldolase. The observations suggest that: (a) hepatocytes from carcinogen-treated liver exhibit the same lack of ability to proliferate in primary culture as normal adult hepatocytes; (b) adult hepatocytes can produce fetal isozymes without prior cell division; (c) pyruvate kinase is a stronger marker of dedifferentiation (retrodifferentiation) than aldolase; and (d) regulatory processes of isozyme expression are different during ontogenesis, regeneration, and hepatocarcinogenesis.
对来自胎儿、再生及经2-乙酰氨基芴处理的大鼠肝脏的培养肝细胞以及一些上皮性肝细胞系中的醛缩酶和丙酮酸激酶同工酶进行了研究。我们的结果表明：(a) 仅在来自17日龄胎儿的培养肝细胞中发现细胞增殖和特定同工酶的延长表达；(b) 在再生肝和经致癌物处理的肝脏中表达的胎儿型丙酮酸激酶仅在来自再生肝的培养肝细胞中暂时缺失；(c) 在源自经致癌物处理的肝脏的一个上皮细胞系和肝癌组织细胞（HTC）系中不存在成人型醛缩酶和丙酮酸激酶，但在Reuber H35细胞系的Faza克隆体外传代的前50代中发现了该同工酶；(d) 丙酮酸激酶的同工酶模式总是比醛缩酶的同工酶模式更强烈地发生改变。这些观察结果表明：(a) 经致癌物处理的肝脏的肝细胞在原代培养中与正常成年肝细胞表现出相同的增殖能力缺乏；(b) 成年肝细胞可以在没有先前细胞分裂的情况下产生胎儿同工酶；(c) 丙酮酸激酶比醛缩酶是去分化（逆分化）的更强标志物；(d) 同工酶表达的调控过程在个体发育、再生和肝癌发生过程中是不同的。
