物种特异性组蛋白H1和H2B基因在小鼠-大鼠杂交细胞中的共表达。

Coexpression of species-specific histone H1 and H2B genes in mouse-rat hybrid cells.

作者信息

Ajiro K, Kano-Tanaka K, Higashida H, Tanaka T

出版信息

Somatic Cell Genet. 1982 May;8(3):377-83. doi: 10.1007/BF01538894.

Abstract

Five different clones of somatic hybrid cell lines between mouse and rat cells were examined for the expression of species-specific histone H1 and H2B subtypes. It was found that one hybrid (140-3) contained only mouse specific histones and the other four clones had both mouse and rat specific histones in various ratios. In three cases (141-B, NBr10A, NBr20A), the relative amount of mouse and rat specific histones was correlated with the karyotype of the hybrids. However, this was not true in one case (NG108-15). The results indicate that a single mammalian hybrid clone can express both parental types of histones, and the histone expression in these hybrids is not regulated by an allelic exclusion but may be expressed codominantly by gene dosage.

摘要

对小鼠和大鼠细胞之间的五种不同体细胞杂交细胞系克隆进行了物种特异性组蛋白H1和H2B亚型表达检测。发现一个杂交细胞系（140-3）只含有小鼠特异性组蛋白，另外四个克隆含有不同比例的小鼠和大鼠特异性组蛋白。在三种情况下（141-B、NBr10A、NBr20A），小鼠和大鼠特异性组蛋白的相对含量与杂交细胞系的核型相关。然而，在一种情况（NG108-15）下并非如此。结果表明，单个哺乳动物杂交克隆可以表达双亲类型的组蛋白，并且这些杂交细胞系中的组蛋白表达不受等位基因排斥的调控，而是可能由基因剂量共显性表达。