[不同物种血清脂蛋白产前状态的电泳研究]

Schlag B, Winkler L, Büttner A, Prange H, Dargel R

Acta Biol Med Ger. 1982;41(2-3):179-85.

The relative proportions of alpha-, beta-, and pre-beta-lipoproteins in the fetal serum of mouse, rat, rabbit, guinea pig, mini-pig, and man were determined by agarose gel electrophoresis and subsequent scan evaluation. No distinct species-independent, fetal-specific common features could be detected. In mouse, rabbit and rat an increase in alpha-lipoproteins and a decrease of the proportion of beta-lipoproteins can be observed during prenatal development. In human cord serum of the 30th week alpha-LP are prevalent; at delivery their proportion is still 50%, like in the serum of adults. Five of the species investigated reveal low, if any, pre-beta-lipoprotein levels in fetal serum, while those in the adult organism amount to 19-33%. The lipoprotein spectrum of the mini-pig at the end of the gestational period is identical with that of the adult animal. In the serum of the fetal and adult guinea pig no alpha-lipoprotein band is detectable. On the other hand, a pre-albumin fraction appeared in the prenatal period, amounting to 25% of total lipoproteins. In total, the findings reported here reflect a largely species-specific development of the fetal lipoprotein patterns.

通过琼脂糖凝胶电泳及随后的扫描评估，测定了小鼠、大鼠、兔子、豚鼠、小型猪和人类胎儿血清中α-脂蛋白、β-脂蛋白和前β-脂蛋白的相对比例。未检测到明显的与物种无关的胎儿特异性共同特征。在小鼠、兔子和大鼠的产前发育过程中，可观察到α-脂蛋白增加，β-脂蛋白比例下降。在第30周的人类脐带血清中，α-LP占主导；分娩时其比例仍为50%，与成人血清中的比例相同。所研究的物种中有5种在胎儿血清中的前β-脂蛋白水平很低（如果有的话），而在成年生物体中则为19%-33%。小型猪在妊娠期结束时的脂蛋白谱与成年动物相同。在胎儿和成年豚鼠的血清中未检测到α-脂蛋白条带。另一方面，在产前出现了一个前白蛋白组分，占总脂蛋白的25%。总体而言，此处报告的研究结果反映了胎儿脂蛋白模式在很大程度上具有物种特异性的发展情况。