Lun A, Pohle R, Schürer M, Gross J
Acta Biol Med Ger. 1982;41(2-3):227-34.
Relations between 59Fe incorporation into red blood cells (rbc) and their density was examined in rats during ontogenesis (20th, 21st, 22nd gestational day, 1st, 21st day of life, adults). The incorporation of 59Fe in rbc of different density was measured 24 or 96 h after application. The density separation was carried out by centrifugation of rbc in phthalate esters of known density. The density of rbc increased during ontogenesis (median density 1.093 kg/l on the 20th gestational day and 1.102 kg/l in adult rats). The erythropoietic activity, in terms of 59Fe-incorporation into peripheral rbc, is higher in newborn rats (60-70%) than in adults (30-50%). 59Fe incorporation into pregnant rats and their fetuses is similar to adult nonpregnant rats. In fetuses a relatively high incorporation of 59Fe was found in comparison to their mother. The relationship between density and maturity of rbc is postnatally more evident than in the prenatal period. (59Fe enrichment in cells of low density prenatally: 2.6 +/- 0.5, postnatally: 8.4 +/- 0.5 (1st day of life), 5.8 +/- 0.8 (21st day of life) 28.5 +/- 5.6 (adult male rats). These findings may reflect a simultaneous formation of rbc at different sites or different clonal origin during prenatal period of ontogenesis.
在大鼠个体发育过程中（妊娠第20、21、22天，出生后第1、21天，成年期），研究了59铁掺入红细胞（RBC）与其密度之间的关系。在给予59铁后24小时或96小时测量不同密度RBC中59铁的掺入情况。通过在已知密度的邻苯二甲酸酯中对RBC进行离心来进行密度分离。在个体发育过程中，RBC的密度增加（妊娠第20天的中位密度为1.093 kg/l，成年大鼠为1.102 kg/l）。就外周RBC中59铁的掺入而言，新生大鼠的红细胞生成活性（60 - 70%）高于成年大鼠（30 - 50%）。妊娠大鼠及其胎儿中59铁的掺入情况与成年未孕大鼠相似。与它们的母亲相比，在胎儿中发现59铁的掺入相对较高。出生后RBC密度与成熟度之间的关系比产前更明显。（产前低密度细胞中59铁富集：2.6 +/- 0.5，产后：出生后第1天为8.4 +/- 0.5，出生后第21天为5.8 +/- 0.8，成年雄性大鼠为28.5 +/- 5.6）。这些发现可能反映了在个体发育的产前阶段，RBC在不同部位同时形成或具有不同的克隆起源。
