Welch W D, Zaccari J
Anesthesiology. 1982 Sep;57(3):172-6. doi: 10.1097/00000542-198209000-00005.
The effect of clinically used concentrations of halothane and N2O on the microbicidal oxidative function of human neutrophils was investigated. Neutrophil oxidative activity was assessed utilizing the method of luminol dependent chemiluminescence (LDCL) by particulate (opsonized zymosn) and nonparticulate (phorbol myristate acetate, [PMA]) stimulated cells. In vivo exposure of neutrophils to 2 and 3% halothane resulted in a 13 and 40% inhibition, respectively of the air-exposed LDCL response with zymosan-activated neutrophils; 1% halothane had no effect. Similar results were seen with PMA-stimulated neutrophils. N2O 80% did not inhibit the LDCL response, and also did not show an additive inhibition when combined with halothane. Although th halothane inhibition of LDCL was reversible (equal to control, no anesthetic, LDCL responses following exposure to air), neutrophils treated with N2O plus halothane and then exposed to air for 30 min showed a significantly higher LDCL response over the control experiments. The inhibition of zymosan- or PMA-stimulated neutrophil LDCL by halothane suggests either a membrane perturbation or a direct inactivation of oxidative enzyme(s) by the anesthetic. This impairment of oxidative activity may partly explain the reduced bacterial killing by neutrophils seen after exposure to halothane.
研究了临床使用浓度的氟烷和一氧化二氮对人中性粒细胞杀菌氧化功能的影响。利用鲁米诺依赖性化学发光法（LDCL），通过颗粒（调理酵母聚糖）和非颗粒（佛波酯，[PMA]）刺激的细胞评估中性粒细胞的氧化活性。中性粒细胞在体内暴露于2%和3%的氟烷，分别导致经酵母聚糖激活的中性粒细胞在暴露于空气时的LDCL反应受到13%和40%的抑制；1%的氟烷没有影响。PMA刺激的中性粒细胞也有类似结果。80%的一氧化二氮不抑制LDCL反应，与氟烷联合使用时也不显示相加抑制作用。虽然氟烷对LDCL的抑制是可逆的（与对照、无麻醉剂、暴露于空气后的LDCL反应相同），但用一氧化二氮加氟烷处理然后暴露于空气30分钟的中性粒细胞，其LDCL反应比对照实验显著更高。氟烷对酵母聚糖或PMA刺激的中性粒细胞LDCL的抑制表明，麻醉剂可能导致膜扰动或氧化酶直接失活。氧化活性的这种损害可能部分解释了暴露于氟烷后中性粒细胞杀菌能力降低的原因。
