Postiglione L, Oriente P, Cimmino F, Nastasi L, Spanò A, Di Fraia G
Boll Soc Ital Biol Sper. 1982 May 30;58(10):605-11.
Enzymatic methods have progressively substituted extractive procedures in plasma lipids determination. A new enzymatic method for serum phospholipids evaluation has been recently introduced in Italy as a kit of reagents. In this method phospolipase D hydrolyzes phospholipids with free choline production which, in presence of choline-oxidase and peroxidase, is estimated by colorimetric reaction. Linearity of response and analytical variations "within-run" and "between-run" during six month observations on serum pools have been preliminarily verified.