[Spectrophotometric study of several rhodamines commonly used in microscopy (1)].

Rhodamines, commonly used in microscopic studies, are basic dyes derived from xanthene. They have recently been used for the quantitative analysis of important cations, as well as in the vital staining of mitochondria of normal or pathological cells and also in the routine staining procedures. These applications may require a knowledge of the spectral behaviour of rhodamines. Here we report the visible and ultraviolet absorption spectra, together with the excitation and fluorescence spectra of the chromatographically pure samples of rhodamine B, rhodamine 6G and violamine R.