Oparina L A
Biokhimiia. 1982 Jun;47(6):1035-8.
Using 7.5% polyacrylamide gel electrophoresis, a heterogeneity of molecular forms of "malic" enzyme (EC 1.1.1.40) in corn leaves was established. Etyolated corn sprouts contain only one component of the enzyme, while in the green leaves 3 minor components were additionally found. A possible existence of the "malic" enzyme in two forms, a compact and a dissociating ones, is postulated. In comparison with the compact form the dissociating form of the enzyme is more active and possesses additional functional properties due to the presence of minor components.
利用7.5%聚丙烯酰胺凝胶电泳法,确定了玉米叶片中“苹果酸”酶(EC 1.1.1.40)分子形式的异质性。黄化玉米芽仅含有该酶的一种成分,而在绿叶中还额外发现了3种次要成分。推测“苹果酸”酶可能以两种形式存在,即紧密型和解离型。与紧密型相比,该酶的解离型更具活性,并且由于次要成分的存在而具有额外的功能特性。