Aprelikova O N, Tomilin N V
Biokhimiia. 1982 Jun;47(6):911-4.
Uracil-DNA-glycosylase which releases uracil residues from DNA has been purified from rat liver more than 300-fold. The enzyme has a molecular weight of about 28,0000, Km = 1.7 . 10(-9) M. The content of uracil-DNA-glycosylase in five different tissues of the rat is correlated with the rate of DNA synthesis in the tissues. The enzyme activity in rat liver increases more than 3 times after partial hepatectomy, showing a peak 28-32 hrs following the surgery. The results obtained suggest that uracil-DNA-glycosylase from mammalian cells releases uracil residues from DNA at time intervals close to those of replication.
从大鼠肝脏中纯化出的尿嘧啶-DNA-糖基化酶，其能从DNA中释放尿嘧啶残基，纯化倍数超过300倍。该酶分子量约为280000，米氏常数Km = 1.7×10⁻⁹M。大鼠五种不同组织中尿嘧啶-DNA-糖基化酶的含量与组织中DNA合成速率相关。大鼠肝脏部分切除术后，该酶活性增加超过3倍，在术后28 - 32小时达到峰值。所得结果表明，来自哺乳动物细胞的尿嘧啶-DNA-糖基化酶在接近复制时间间隔时从DNA中释放尿嘧啶残基。
