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从牛胎盘组织和子宫中分离并对富含半胱氨酸的胶原蛋白进行天然特性分析及其与IV型和V型胶原蛋白的关系

Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens.

作者信息

Abedin M Z, Ayad S, Weiss J B

出版信息

Biosci Rep. 1982 Jul;2(7):493-502. doi: 10.1007/BF01115247.

DOI:10.1007/BF01115247
PMID:7115902
Abstract

A simplified procedure for the fractionation and purification of different collagen types from various tissues is described which is particularly efficient in separating type-V from type-IV collagen, and high-mol.-wt. (HMW) aggregates from 7 S collagen. Uterus and maternal villi contain 2 forms of type-V collagen - [alpha 1(V)]2 alpha 2(V) and [alpha 1(V) alpha 2(V) alpha 3(V) - which have been separated on DEAE-cellulose. Uterus however appears to be the richest source of both HMW aggregates and the [alpha 1(V) alpha 2(V) alpha 3(V)] collagen, and a probable relationship between these collagens is discussed.

摘要

本文描述了一种从各种组织中分离和纯化不同类型胶原蛋白的简化方法，该方法在从IV型胶原蛋白中分离V型胶原蛋白以及从7S胶原蛋白中分离高分子量（HMW）聚集体方面特别有效。子宫和母体绒毛含有两种形式的V型胶原蛋白——[α1(V)]2α2(V)和[α1(V)α2(V)α3(V)]——它们已在DEAE-纤维素上分离。然而，子宫似乎是HMW聚集体和[α1(V)α2(V)α3(V)]胶原蛋白最丰富的来源，并讨论了这些胶原蛋白之间可能的关系。

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1
Isolation and native characterization of cysteine-rich collagens from bovine placental tissues and uterus and their relationship to types IV and V collagens.从牛胎盘组织和子宫中分离并对富含半胱氨酸的胶原蛋白进行天然特性分析及其与IV型和V型胶原蛋白的关系
Biosci Rep. 1982 Jul;2(7):493-502. doi: 10.1007/BF01115247.
2
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Characterization of the six zebrafish clade B fibrillar procollagen genes, with evidence for evolutionarily conserved alternative splicing within the pro-alpha1(V) C-propeptide.
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