[14C]重氮丝氨酸与大鼠和仓鼠体内DNA及蛋白质的结合

Binding of [14C] azaserine to DNA and protein in the rat and hamster.

作者信息

Zurlo J, Coon C I, Longnecker D S, Curphey T J

出版信息

Cancer Lett. 1982 May-Jun;16(1):65-70. doi: 10.1016/0304-3835(82)90092-1.

Abstract

The binding of [14C] azaserine or its metabolites to DNA and protein in the organs of rats and hamsters was determined at various time after treatment with [14C] azaserine. The specific activity of 14C labelling of DNA and protein was determined. Rat liver DNA and protein were most extensively labelled at 90 min post-injection, but by 24 h the specific activity decreased to the levels found in pancreas and kidney. Thymus contained negligible amounts of radioactivity at all time-points. DNA and protein from hamster pancreas contained more label than did DNA and protein from rat pancreas. The results suggest that factors other than DNA binding play a role in determining the species and organ specificity of azaserine.

摘要

在用[¹⁴C]重氮丝氨酸处理后的不同时间，测定了[¹⁴C]重氮丝氨酸或其代谢产物与大鼠和仓鼠器官中DNA和蛋白质的结合情况。测定了DNA和蛋白质的¹⁴C标记比活度。注射后90分钟时，大鼠肝脏DNA和蛋白质的标记最为广泛，但到24小时时，比活度降至胰腺和肾脏中的水平。在所有时间点，胸腺中的放射性含量均可忽略不计。仓鼠胰腺的DNA和蛋白质比大鼠胰腺的DNA和蛋白质含有更多的标记物。结果表明，除了与DNA结合外，其他因素在决定重氮丝氨酸的物种和器官特异性方面也起作用。