棕色固氮菌铁氧化还原蛋白I的高电位和低电位4Fe-4S*中心的电位滴定
Potentiometric titration of the high- and low-potential 4Fe-4S* centers of Azotobacter vinelandii ferredoxin I.
作者信息
Yoch D C, Carithers R P
出版信息
J Bacteriol. 1978 Nov;136(2):822-4. doi: 10.1128/jb.136.2.822-824.1978.
Abstract
The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using the absorbance decrease at 410 nm to monitor its reduction. This center exhibited an Em of -424 mV (n = 1).
摘要
通过还原程序对棕色固氮菌铁氧化还原蛋白I的高电位4Fe-4S中心进行了电位滴定。在用Em为320 mV（n = 1）的高电位中心滴定过程中，510 nm处的吸光度下降。通过利用410 nm处的吸光度下降来监测其还原，对低电位4Fe-4S中心进行了滴定。该中心的Em为-424 mV（n = 1）。
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