Gotoh M, Mochizuki Y, Furukawa K, Sawada N, Tsukada H
Gan. 1982 Feb;73(1):14-20.
Histochemical and cytochemical studies were done to further characterize the preneoplastic hepatocytes with extraperoxisomal catalase (EPC-cells) that were found in the livers of rats fed 3'-methyl-4-dimethylaminoazobenzene. Catalase activity was demonstrated cytochemically to be localized in nuclear matrices, hyaloplasm, and peroxisomal matrices of EPC-cells. It is suggested that an impairment of peroxisome formation is involved in the altered intracellular distribution of catalase. Administration of clofibrate increased the catalase activity in EPC-cells. To examine the preneoplastic nature of EPC-cells, activities of glucose-6-phosphatase (G6Pase) and gamma-glutamyl transpeptidase (GGT) were examined, since changes in the activities of these enzymes have been used as markers of putative preneoplastic cells. In the present study, neither weak G6Pase activity nor positive GGT activity was considered to be a consistent feature of EPC-cells. However, available evidence suggests that EPC-cells are one of the carcinogen-induced cell populations with altered phenotypes.
进行了组织化学和细胞化学研究，以进一步表征在喂食3'-甲基-4-二甲基氨基偶氮苯的大鼠肝脏中发现的具有过氧化物酶体催化酶（EPC细胞）的癌前肝细胞。通过细胞化学方法证明，过氧化氢酶活性定位于EPC细胞的核基质、透明质和过氧化物酶体基质中。提示过氧化物酶体形成受损与过氧化氢酶细胞内分布改变有关。氯贝丁酯的给药增加了EPC细胞中的过氧化氢酶活性。为了研究EPC细胞的癌前性质，检测了葡萄糖-6-磷酸酶（G6Pase）和γ-谷氨酰转肽酶（GGT）的活性，因为这些酶活性的变化已被用作假定癌前细胞的标志物。在本研究中，弱G6Pase活性和阳性GGT活性均未被认为是EPC细胞的一致特征。然而，现有证据表明，EPC细胞是具有表型改变的致癌物诱导细胞群体之一。
