Park Y H, Igimi H, Carey M C
Gastroenterology. 1982 Nov;83(5):1071-8.
We describe a novel "silvering" method to determine the surface area of nonspherical objects and have applied this method to human cholesterol gallstones. A standard curve is first constructed, using data obtained from the dissolution rates of silver-coated plastic spheres of known dimensions in unstirred thiosulfate-ferricyanide solutions. Silver dissolution rates of silver-coated gallstones are then correlated with the standard curve so that the equivalent spherical surface area of the gallstones may be estimated. The technique has been applied to cholesterol dissolution rates of gallstones from the same patient and from different patients in unstirred bile salt (sodium chenodeoxycholate) solutions. The results demonstrate that cholesterol dissolution rates per unit surface area are identical for small stones as well as large ones, are identical for all stones from the same gallbladder, but are somewhat dissimilar for those originating from different gallbladders. The experimental results are in agreement with a priori theoretical predictions of mass transfer from spherical objects, and correlate satisfactorily with experimental dissolution rates of pure cholesterol monohydrate disks in unstirred bile salt solutions.
我们描述了一种用于确定非球形物体表面积的新型“镀银”方法，并已将此方法应用于人体胆固醇胆结石。首先构建标准曲线，使用从已知尺寸的镀银塑料球在未搅拌的硫代硫酸盐 - 铁氰化物溶液中的溶解速率获得的数据。然后将镀银胆结石的银溶解速率与标准曲线相关联，以便估计胆结石的等效球表面积。该技术已应用于在未搅拌的胆汁盐（鹅去氧胆酸钠）溶液中来自同一患者和不同患者的胆结石的胆固醇溶解速率。结果表明，每单位表面积的胆固醇溶解速率对于小结石和大结石是相同的，对于来自同一胆囊的所有结石是相同的，但对于来自不同胆囊的结石则有些不同。实验结果与球形物体传质的先验理论预测一致，并且与未搅拌的胆汁盐溶液中纯胆固醇一水合物盘的实验溶解速率令人满意地相关。
