Kuettner K E, Memoli V A, Pauli B U, Wrobel N C, Thonar E J, Daniel J C
J Cell Biol. 1982 Jun;93(3):751-7. doi: 10.1083/jcb.93.3.751.
The in vitro phenotype of bovine articular chondrocytes is described. Chondrocytes plated at high density in roller-bottle and dish cultures were maintained in vitro. The major matrix macromolecules, collagen and proteoglycan, synthesized by these cells were characterized during the course of the culture period. The chondrocytes synthesized mainly Type II collagen, which was found predominantly in the cell-associated matrix. The media contained a mixture of Type II and Type III collagens. Type I collagen was detectable in neither the medium nor the cell-associated matrix. The proteoglycan monomers found in media and cell-associated matrix had the same hydrodynamic sizes as monomers synthesized by cartilage slices or those extracted from adult articular cartilage. The majority of proteoglycans synthesized by the cells were found in high molecular weight aggregates which were readily recovered from the media and were extractable from cell-associated matrix with low ionic strength buffers. The results demonstrate the long-term in vitro phenotypic stability of the bovine articular chondrocytes. The advantages of the in vitro system as a model for studying the effects of external agents, such as drugs and vitamins, are discussed.
本文描述了牛关节软骨细胞的体外表型。将高密度接种于滚瓶和平皿培养物中的软骨细胞进行体外培养。在培养期间，对这些细胞合成的主要基质大分子——胶原蛋白和蛋白聚糖进行了表征。软骨细胞主要合成II型胶原蛋白，其主要存在于细胞相关基质中。培养基中含有II型和III型胶原蛋白的混合物。在培养基和细胞相关基质中均未检测到I型胶原蛋白。在培养基和细胞相关基质中发现的蛋白聚糖单体的流体动力学大小与软骨切片合成的单体或从成年关节软骨中提取的单体相同。细胞合成的大多数蛋白聚糖存在于高分子量聚集体中，这些聚集体很容易从培养基中回收，并且可用低离子强度缓冲液从细胞相关基质中提取。结果证明了牛关节软骨细胞在体外的长期表型稳定性。讨论了该体外系统作为研究药物和维生素等外部因素作用模型的优点。
