Zighelboim J, Bick A, Durantez A
Cancer Res. 1977 Oct;37(10):3656-62.
A human serum (obtained from a multiparous and multiple-transfused patient with chronic myelogenous leukemia) and a rabbit antiserum (obtained by immunization with papain extracts from a B-lymphoblastoid cell line) showed reactivity against antigenic specificities (different from HLA) expressed on peripheral blood B-lymphocytes, unmarked lymphocytes, and monocytes. These antigenic determinants were expressed on myeloblasts and lymphoblasts from patients with acute leukemia (during the active phase of their disease) and on B-lymphoblastoid cell lines and lymphocytes from patients with chronic lymphocytic leukemia. Purified peripheral blood T-lymphocytes, mitogen (phytohemagglutinin)-activated T-lymphocytes, and lymphoblasts (with T-cell characteristics) obtained from patients with acute lymphoblastic leukemia or established lymphoblastoid cell lines lacked these antigenic specificities. Absorption experiments indicate that the antigen(s) detected on normal mononuclear cell populations, leukemia cells, and B-lymphoblastoid cell lines were either identical or highly cross-reactive.
一份人血清（取自一位患有慢性粒细胞白血病的经产妇且多次输血的患者）和一份兔抗血清（通过用来自B淋巴母细胞系的木瓜蛋白酶提取物免疫获得）显示出与外周血B淋巴细胞、未标记淋巴细胞和单核细胞上表达的抗原特异性（不同于HLA）发生反应。这些抗原决定簇在急性白血病患者（在疾病的活跃期）的成髓细胞和淋巴母细胞上表达，以及在慢性淋巴细胞白血病患者的B淋巴母细胞系和淋巴细胞上表达。纯化的外周血T淋巴细胞、丝裂原（植物血凝素）激活的T淋巴细胞以及从急性淋巴细胞白血病患者获得的具有T细胞特征的淋巴母细胞或已建立的淋巴母细胞系缺乏这些抗原特异性。吸收实验表明，在正常单核细胞群体、白血病细胞和B淋巴母细胞系上检测到的抗原要么相同，要么具有高度交叉反应性。
