Koshiba H, Minowada J, Pressman D
J Natl Cancer Inst. 1978 Oct;61(4):987-91.
Rabbit immune sera against human cells of a non-T, non-B leukemia cell line bearing the Ph1 chromosome (NALM-1) were characterized. After proper and exhaustive absorption, the sera were tested by indirect membrane immunofluorescence on a panel of 19 human hematopoietic cell lines with T-, B-, or non-T, non-B cell surface characteristics. The absorbed sera reacted specifically with NALM-1 cells but not with cells of another Ph1-positive cell line, K-562. Four of the 6 leukemia T-cell lines, 2 of the 4 leukemia-lymphoma B-cell lines, all of 4 acute lymphoblastic leukemia (ALL) lines with non-T, non-B cell characteristics, and uncultured cells of all patients with non-T, non-B cell ALL were reactive with the antisera. A cross-absorption study of the antisera suggested that a single antigenic complex is involved in this antigen specificity. The antigen involved appears to be a common non-T, non-B ALL one that has been described previously.
对携带费城染色体（Ph1）的非T、非B白血病细胞系（NALM-1）的人细胞的兔免疫血清进行了特性鉴定。经过适当且充分的吸收后，通过间接膜免疫荧光法在一组具有T细胞、B细胞或非T、非B细胞表面特征的19种人造血细胞系上对血清进行检测。吸收后的血清与NALM-1细胞发生特异性反应，但不与另一种Ph1阳性细胞系K-562的细胞反应。6种白血病T细胞系中的4种、4种白血病淋巴瘤B细胞系中的2种、所有4种具有非T、非B细胞特征的急性淋巴细胞白血病（ALL）细胞系以及所有非T、非B细胞ALL患者的未培养细胞均与抗血清发生反应。抗血清的交叉吸收研究表明，这种抗原特异性涉及单一抗原复合物。所涉及的抗原似乎是一种先前已描述过的常见的非T、非B ALL抗原。
