神经内分泌学中的重组DNA方法：旧问题的新答案

Recombinant DNA methods in neuroendocrinology: new answers to old questions.

作者信息

Eiden L E

出版信息

Peptides. 1982 May-Jun;3(3):217-21. doi: 10.1016/0196-9781(82)90081-x.

Abstract

The development of methods for cloning DNA complementary to specific messenger RNAs encoding polypeptide hormones has opened new experimental avenues in the neurosciences. Recombinant DNA techniques have been used to answer questions about the structure of polypeptide hormone precursors, the regulation of peptide hormone production, and the location of peptide-producing cells in nervous and endocrine systems. Here, the application of cDNA cloning to the quantitation, structural elucidation and tissue localization of mRNAs coding for neuroendocrine peptides is reviewed.

摘要

克隆与编码多肽激素的特定信使核糖核酸互补的DNA方法的发展，为神经科学开辟了新的实验途径。重组DNA技术已被用于解答有关多肽激素前体结构、肽激素产生的调节以及神经和内分泌系统中产生肽的细胞位置等问题。本文综述了cDNA克隆在神经内分泌肽编码信使核糖核酸的定量、结构解析和组织定位方面的应用。