Lee H Y, Nagele R G, Goldstein M M
J Exp Zool. 1978 Dec;206(3):457-62. doi: 10.1002/jez.1402060315.
Primordial germ cells (PGCs) of the early chick embryo were examined using scanning electron microscopy. Temporal changes in the form and distribution of surface projections were found to be correlated with migratory phases of PGCs. Non-migrating PGCs were spherical to ovoid with relatively smooth surfaces. Their transition to the migratory phase was first evidenced by a burst of membrane activity. Migrating PGCs became somewhat flattened against the underlying hypoblast (which serves as the substratum for their migration) and exhibited blebs and lamellar processes. The lamellar processes were most prominent at the leading edges of actively migrating PGCs. Overall results of the present study indicates that PGCs found in the germinal crescent area of early chick embryos actively migrate on the dorsal surface of the hypoblast towards posterior embryonic regions.
利用扫描电子显微镜对早期鸡胚的原始生殖细胞（PGCs）进行了检查。发现表面突起的形态和分布的时间变化与PGCs的迁移阶段相关。未迁移的PGCs呈球形至卵形，表面相对光滑。它们向迁移阶段的转变首先表现为膜活性的爆发。迁移的PGCs在下方的内胚层（作为其迁移的基质）上变得有些扁平，并表现出泡状和片状突起。片状突起在活跃迁移的PGCs的前缘最为突出。本研究的总体结果表明，在早期鸡胚生殖新月区发现的PGCs在胚盘背面向胚胎后部区域积极迁移。
